Anti-GFAP delta 抗体 (ab93251)
Key features and details
- Rabbit polyclonal to GFAP delta
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GFAP delta antibody -
製品の詳細
Rabbit polyclonal to GFAP delta -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab68428.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Mouse GFAP delta aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab129227) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Cerebellum; Mouse Hippocampus; Mouse Spinal Cord. This antibody gave a positive result in IHC in the following FFPE tissue: Normal mouse adult brain.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93251の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IHC-P | (2) |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
- Unigene: 1239 Mouse
- Unigene: 91512 Rat
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別名
- GFAP antibody
- GFAP Epsilon antibody
- GFAP_HUMAN antibody
see all
画像
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IHC image of GFAP delta staining in normal Mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93251, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
All lanes : Anti-GFAP delta antibody (ab93251) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Cerebellum Mouse Tissue Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 15 kDa, 47 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab93251 は 3 報の論文で使用されています。
- Li Y et al. Prenatal ethanol exposure impairs the formation of radial glial fibers and promotes the transformation of GFAPd-positive radial glial cells into astrocytes. Mol Med Rep 23:N/A (2021). PubMed: 33576465
- Gómez-Gálvez Y et al. Quantitative proteomic profiling of the rat substantia nigra places glial fibrillary acidic protein at the hub of proteins dysregulated during aging: Implications for idiopathic Parkinson's disease. J Neurosci Res 98:1417-1432 (2020). PubMed: 32270889
- Liu JYW et al. Doublecortin-expressing cell types in temporal lobe epilepsy. Acta Neuropathol Commun 6:60 (2018). PubMed: 30005693