Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory stem cells
Blocking step
BSA 3% + Goat serum 5% as blocking agent for 1 hour as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Diluent: Blocking solution without Trtion
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Native from an ectodermic tissue, olfactory stem cell express GFAP after spheres induction.
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投稿 Oct 25 2016