Alexa Fluor® 647 Anti-GFAP 抗体 [EPR1034Y] (ab194325)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR1034Y] to GFAP
- Suitable for: IHC-Fr
- Reacts with: Rat
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y]
GFAP 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 647 Rabbit monoclonal [EPR1034Y] to GFAP -
由来種
Rabbit -
標識
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
アプリケーション
適用あり: IHC-Frmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-Fr: Rat brain (Hypothalamus).
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1034Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324)
- Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] (ab201732)
- Alexa Fluor® 555 Anti-GFAP antibody [EPR1034Y] (ab201735)
- Alexa Fluor® 568 Anti-GFAP antibody [EPR1034Y] (ab201736)
- Alexa Fluor® 405 Anti-GFAP antibody [EPR1034Y] (ab206586)
- Anti-GFAP antibody [EPR1034Y] - BSA and Azide free (ab218309)
- Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric (ab313596)
- Biotin Anti-GFAP antibody [EPR1034Y] (ab314264)
- Anti-GFAP antibody [EPR1034Y] (ab68428)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab194325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr |
1/100 - 1/500.
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特記事項 |
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IHC-Fr
1/100 - 1/500. |
ターゲット情報
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機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
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参照データベース
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
- Unigene: 514227 Human
see all -
別名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
画像
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IHC image of ab194325 staining in 10% formaldehyde fixed frozen tissue section of normal rat brain.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab194325 (1/100 dilution) and DAPI in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab194325.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab194325 は 1 報の論文で使用されています。
- Guo YH et al. Effects of ginsenoside Rg1-loaded alginate-chitosan microspheres on human bone marrow stromal cells. Biosci Rep 37:N/A (2017). PubMed: 28536312