Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293 cells)
Total protein in input
1000 µg
Specification
293 cells
Immuno-precipitation step
Other - protein G dynabeads
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Name
Abcam antibody:(not specified yet)
Dilution
1/100
Additional data
Additional Notes
Lane 1 shows 30 µg of total 293 cell extract as input, lane 3 shows the IP. Extracts where prepared with 450 mM NaCl, then diluted to 150 mM NaCl for the IP. We used 20 µl of GEF H1 antibody and incubated with 1 mg of total 293 cell extract for 2 hours. Then protein G dynabeads were added according to the manufacturers instructions for an additional hour. Samples were analysed by SDS PAGE and western blotting using GEF H1 antibody at a dilution of 1:100.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Melanie Blasius
Verified customer
投稿 Nov 26 2010