Anti-GDF6 抗体 (ab73288)
Key features and details
- Rabbit polyclonal to GDF6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GDF6 antibody
GDF6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GDF6 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
A 17 amino acid peptide near the carboxy terminus of human GDF6.
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ポジティブ・コントロール
- SK-N-SH lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Growth factor that controls proliferation and cellular differentiation in the retina and bone formation. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map (PubMed:23307924). Required for normal formation of bones and joints in the limbs, skull, digits and axial skeleton. Plays a key role in establishing boundaries between skeletal elements during development. Regulation of GDF6 expression seems to be a mechanism for evolving species-specific changes in skeletal strucutres. Seems to positively regulates differentiation of chondrogenic tissue through the growth factor receptors subunits BMPR1A, BMPR1B, BMPR2 and ACVR2A, leading to the activation of SMAD1-SMAD5-SMAD8 complex. The regulation of chondrogenic differentiation is inhibited by NOG (PubMed:26643732). Also involved in the induction of adipogenesis from mesenchymal stem cells. This mechanism acts through the growth factor receptors subunits BMPR1A, BMPR2 and ACVR2A and the activation of SMAD1-SMAD5-SMAD8 complex and MAPK14/p38. -
関連疾患
Klippel-Feil syndrome 1, autosomal dominant
A chromosomal aberration involving GDF6 has been found in a patient with Klippel-Feil syndrome (KFS). Paracentric inv(8)(q22;2q23.3).
Microphthalmia, isolated, 4
Leber congenital amaurosis 17
Defects in POP1 may be the cause of multiple synostoses syndrome (SYNS). SYNS is a bone disease characterized by multiple progressive joint fusions that commonly involve proximal interphalangeal, tarsal-carpal joints. Additional features can include progressive conductive deafness. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 392255 Human
- Omim: 601147 Human
- SwissProt: Q6KF10 Human
- Unigene: 492277 Human
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別名
- bmp 13 antibody
- BMP-13 antibody
- bmp13 antibody
see all
画像
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Lane 1 : Anti-GDF6 antibody (ab73288) at 0.5 µg/ml
Lane 2 : Anti-GDF6 antibody (ab73288) at 1 µg/ml
All lanes : SK-N-SH lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
All lanes : Anti-GDF6 antibody (ab73288) at 1 µg/ml
Lane 1 :Recombinant Human GDF6 protein (ab50230) at 0.1 µg
Lane 2 :Recombinant Human GDF6 protein (ab50230) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Exposure time: 30 seconds
ab50230 is a homodimer consisting of two 120aa monomers. The homodimer format is expected to run at 27kDa, so the observed band is thought to be the monomer form.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab73288 は 2 報の論文で使用されています。
- Jiang F et al. Construction of mRNA prognosis signature associated with differentially expressed genes in early stage of stomach adenocarcinomas based on TCGA and GEO datasets. Eur J Med Res 27:205 (2022). PubMed: 36253873
- Harrison CB et al. Fibroblast Nox2 (NADPH Oxidase-2) Regulates ANG II (Angiotensin II)-Induced Vascular Remodeling and Hypertension via Paracrine Signaling to Vascular Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:698-710 (2021). PubMed: 33054395