Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% gel)
Sample
Human Cell lysate - whole cell (HELA)
Specification
HELA
Treatment
10uM Tunicamycin for 24hrs
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: DONKEY-ANTI-GOAT
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 35 kDa Non-specific: 36, 40, 52, 53, 60, 80, 98 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Lane 1: Protein Ladder;
Lanes 2,3,4: Control HELA cells;
Lane 5: HELA cells treated with TUNICAMYCIN.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 30 2013