Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Cell Line Human Lung Carcinoma?)
Gel Running Conditions
Reduced Denaturing (6% SDS-PAGE)
Loading amount
15 µg
Specification
A549 Cell Line Human Lung Carcinoma?
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% BSA in PBS
Dilution
1/2500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: between 55 and 35 kD kDa Non-specific: between 72 and 55 kD kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 20 2016