Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Mouse tracheal tissue)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Mouse tracheal tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in 0.1% PBST
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey-anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
Thank you for your valuable feedback. We are pleased to see that the antibody worked well for you in IHC-Fr, an application that has not been experimentally tested before. We welcome any further feedback and Abreviews from other customers who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Armen Petrosyan
Verified customer
投稿 Jan 20 2016