Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
No
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were MeOH fixed, immunofluorescently labeled and counterstained with DAPI. This antibody produces an extremely weak staining pattern. PF fixed samples were also evaluated failed to produce a specific staining pattern.
Abcam response
Thank you for your valuable feedback. Your data suggests that ab62363 may not be suitable to detect endogenous GCDFP by ICC/IF in HeLa cells under these experimental conditions. We hope this information is useful to our customers and we welcome further feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jan 28 2014