GAS 6 overexpression 293T lysate (whole cell) (ab94148)
製品の概要
-
製品名
GAS 6 overexpression 293T lysate (whole cell)
GAS 6 ライゼート 製品一覧 -
特記事項
ab94148 is a 293T cell transfected lysate in which Human GAS 6 has been transiently over-expressed using a pCMV-GAS 6 plasmid. The lysate is provided in 1X Sample Buffer.
-
アプリケーション
適用あり: WBmore details
製品の特性
-
Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究分野
-
背景
Function: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Tissue specificity: Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. Similarity: Contains 4 EGF-like domains. Contains 1 Gla (gamma-carboxy-glutamate) domain. Contains 2 laminin G-like domains. PTM: Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
関連製品
-
Corresponding Antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94148の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
特記事項 |
---|
WB
Use at an assay dependent dilution. |
画像
-
ab94148 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-GAS 6 antibody (ab67099) at 1/500 dilution
Lane 1 :GAS 6 overexpression 293T lysate (whole cell) (ab94148)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP comjugated at 1/2500 dilution
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab94148 は論文での使用が確認できていません。