Anti-GARS 抗体 [EPR7157] (ab125008)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7157] to GARS
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-GARS antibody [EPR7157]
GARS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7157] to GARS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human GARS aa 700-800 (C terminal). The exact sequence is proprietary.
-
ポジティブ・コントロール
- Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7157 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125008の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
関連性
GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease. -
細胞内局在
Cytoplasm. Mitochondrion. -
参照データベース
- Entrez Gene: 2617 Human
- Entrez Gene: 353172 Mouse
- Entrez Gene: 297113 Rat
- Omim: 600287 Human
- SwissProt: P41250 Human
- SwissProt: Q9CZD3 Mouse
- SwissProt: Q5I0G4 Rat
-
別名
- AP 4 A synthetase antibody
- Charcot Marie Tooth neuropathy 2D antibody
- Charcot Marie Tooth neuropathy neuronal type D antibody
see all
画像
-
All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab125008 は論文での使用が確認できていません。