製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GARNL3
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    amino acids 737-828 (LVQPSASDFQ FCWNQAPYAI VCAFPYLLAF TTDSMEIRLV VNGNLVHTAV VPQLQLVASR SDIYFTATAA VNEVSSGGSS KGASARNSPQ TP) of Human GARNL3 (Q5VVW2).

  • ポジティブ・コントロール
    • Human nasopharynx tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

画像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles. Recommended concentration of ab122015 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122015 staining GARNL3 in paraffin-embedded Human nasopharynx tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

参考文献

ab122015 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab122015.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録