Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Liver, RPE, retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Liver, RPE, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor Odyssey Clx
Exposure
15 minute(s) and 0 second(s)
Positive control
HEK cell lysate, mouse liver lysate
Additional data
Additional Notes
I was previously told to mark inconclusive in a blot for an application that is not guaranteed or purported (e.g. for zebrafish) if this is not the case, change to 1.
Lane 1: HEK cells
Lane 2: hfRPE cells
Lane 3: hfRetina
Lane 4: Mouse liver
Lane 5: Mouse RPE
Lane 6: Mouse retina
Lane 7: Zebrafish liver
Lane 8: Zebrafish retina
PVDF membrane
RIPA lysis buffer with mixed phosphatase and protease inhibitor cocktail
Abcam response
The antibody is guaranteed to work with Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Xenopus laevis, Cynomolgus monkey, Chinese hamster species. As lanes 1 and 2 indicate - human works and lanes 5 and 6 show - mouse works. The zebrafish sample which customer was testing, unfortunately did not work.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 24 2017