Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Non-reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Dilution
1/8000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 17°C · Diluent: 1% BSA
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 36 kDa Non-specific: No kDa
Positive control
cytosolic cell lysate
Negative control
Nuclear cell lysate
Additional data
Additional Notes
Fantastic antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 30 2012