Application
Western blot
Sample
Neurospora crassa Cell lysate - whole cell (mycelium)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
1 µg
Specification
mycelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% Milk in TBST
Dilution
1/4000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Immobilon ECL
Exposure
20 second(s)
Bands
Specific: 45 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 22 2016