Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (16%)
Sample
Mouse Cell lysate - whole cell (Primary bone marrow derived macrophage)
Specification
Primary bone marrow derived macrophage
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in PBST
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 37 kDa
Exposure
2 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 11 2014