Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS + 0.3 % Triton X100
Secondary antibody
Name
Non-Abcam antibody was used: Chicken anti-goat Cy3
Host species: Chicken
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Chicken
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/300
Additional data
Additional Notes
The antibody stains the membrane of many cells in the mouse brain.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Jul 31 2009