Application
Western blot
Sample
Human Cell lysate - whole cell (human whole cell lysate)
Loading amount
10 µg
Specification
human whole cell lysate
Gel Running Conditions
Reduced Denaturing (4-12% PA gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 39 kDa
Additional data
Additional Notes
1 - HeLa whole cell lysate
2 - H1299 whole cell lysate
2 - H1299 whole cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
投稿 Jan 12 2011