Anti-GAPDH 抗体 [GA1R] - Loading Control (ab125247)
Key features and details
- Mouse monoclonal [GA1R] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
- Isotype: IgG1
製品の概要
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製品名
Anti-GAPDH antibody [GA1R] - Loading Control
GAPDH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GA1R] to GAPDH - Loading Control -
由来種
Mouse -
特異性
ab125247 recognizes native and denatured forms of GAPDH. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Escherichia coli, Spodoptera frugiperda (SF9 cells)
交差が予測される動物種: a wide range of other species -
免疫原
Recombinant full length protein corresponding to Rabbit GAPDH.
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ポジティブ・コントロール
- Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
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特記事項
Store at 4°C (add 0.05% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Protein A affinity chromatography from TCS -
ポリ/モノ
モノクローナル -
クローン名
GA1R -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (7) |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
with ECL. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL. |
ターゲット情報
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機能
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
配列類似性
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
翻訳後修飾
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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参照データベース
- Entrez Gene: 374193 Chicken
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Omim: 138400 Human
see all -
別名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
画像
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All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : Human tissue lysate
Lane 2 : Mouse tissue lysate
Lane 3 : Rat tissue lysate
Lane 4 : Rabbit tissue lysate
Lane 5 : Chicken tissue lysate
Lane 6 : Hamster tissue lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa -
All lanes : Anti-GAPDH antibody [GA1R] - Loading Control (ab125247) at 1/2000 dilution
Lane 1 : BL21 bacterial lysate
Lane 2 : Sf9 insect lysate
Lane 3 : Saccharomyces cerevisiae lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (116)
ab125247 は 116 報の論文で使用されています。
- Deng H et al. Identification and Validation of N6-Methyladenosine-Related Biomarkers for Bladder Cancer: Implications for Immunotherapy. Front Oncol 12:820242 (2022). PubMed: 35311150
- Wei W et al. circ_0020123 promotes cell proliferation and migration in lung adenocarcinoma via PDZD8. Open Med (Wars) 17:536-549 (2022). PubMed: 35415250
- Harmuth T et al. Mitochondrial Dysfunction in Spinocerebellar Ataxia Type 3 Is Linked to VDAC1 Deubiquitination. Int J Mol Sci 23:N/A (2022). PubMed: 35682609
- Conan P et al. Identification of 8-Hydroxyquinoline Derivatives That Decrease Cystathionine Beta Synthase (CBS) Activity. Int J Mol Sci 23:N/A (2022). PubMed: 35743210
- Li Y et al. Efficacy of bivalent CEACAM6/4-1BBL genetic vaccine combined with anti-PD1 antibody in MC38 tumor model of mice. Heliyon 8:e10775 (2022). PubMed: 36212004