Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse whole brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mouse whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% nonfat milk in TBS +0.1%tween
Dilution
1/100000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL prime
Exposure
3 second(s)
Bands
Specific: 43 kDa
Additional data
Additional Notes
Excellent antibody for westerns. Super strong- don't overload total protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 04 2017