Anti-GALNT8 抗体 (ab121374)
Key features and details
- Rabbit polyclonal to GALNT8
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GALNT8 antibody -
製品の詳細
Rabbit polyclonal to GALNT8 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human GALNT8 aa 502-629.
Database link: Q9NY28 -
ポジティブ・コントロール
- WB: RT4 and U251 MG cell lysates. ICC: U-2 OS cells. IHC-P: Human testis, rectum and cerebellum tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Probably catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. -
組織特異性
Widely expressed. Expressed in heart, skeletal muscle, kidney, liver, small intestine and placenta. Weakly expressed in colon, thymus, spleen, lung and leukocyte. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.
Contains 1 ricin B-type lectin domain. -
ドメイン
There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26290 Human
- Omim: 606250 Human
- SwissProt: Q9NY28 Human
- Unigene: 511985 Human
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別名
- GALNAC T8 antibody
- GalNAc-T8 antibody
- GALNT8 antibody
see all
画像
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Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles. Antibody staining is shown in green.
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All lanes : Anti-GALNT8 antibody (ab121374) at 1/250 dilution
Lane 1 : RT4 lysate
Lane 2 : U251 MG lysate
Developed using the ECL technique. -
Immunohistochemical analysis of human testis tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
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Immunohistochemical analysis of human rectum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
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Immunohistochemical analysis of human cerebellum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
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Immunohistochemical analysis of human liver tissue labeling GALNT8 with ab121374 at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab121374 は論文での使用が確認できていません。