Application
Western blot
Sample
Rat Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Specification
whole brain
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/5000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: rabbit anti-mouse
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
secondary alone
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 67 kDa Non-specific: none kDa
Positive control
Brain is positive control. Only single strong band was observed at 67 kD, as expected.
Additional data
Additional Notes
Very clean antibody (see photo)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Brian Pham
Verified customer
投稿 Jan 05 2018