Application
Western blot
Sample
Human Tissue lysate - whole (skeletal muscle tissue)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
skeletal muscle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + Milk 1%
Dilution
1/500
Secondary antibody
Dilution
1/7000
Name
Non-Abcam antibody was used: anti-rabbit IgG (whole molecule)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 14 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 12 2015