Anti-GABA B Receptor 2/GABBR2 抗体 (ab52248)
Key features and details
- Rabbit polyclonal to GABA B Receptor 2/GABBR2
- Suitable for: IHC-FoFr, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-GABA B Receptor 2/GABBR2 antibody
GABA B Receptor 2/GABBR2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GABA B Receptor 2/GABBR2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-FoFr, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human GABA B Receptor 2/GABBR2.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52248の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
WB |
1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
|
特記事項 |
---|
IHC-FoFr
Use at an assay dependent concentration. |
WB
1/300 - 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
ターゲット情報
-
機能
Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. -
組織特異性
Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle. -
配列類似性
Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. -
ドメイン
Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 9568 Human
- Entrez Gene: 83633 Rat
- Omim: 607340 Human
- SwissProt: O75899 Human
- SwissProt: O88871 Rat
- Unigene: 198612 Human
- Unigene: 162814 Rat
-
別名
- BcDNA:GH07312 antibody
- CG6706 antibody
- CT20836 antibody
see all
画像
-
All lanes : Anti-GABA B Receptor 2/GABBR2 antibody (ab52248) at 1/300 dilution
Lane 1 : HeLa cell extract; without peptide
Lane 2 : HeLa cell extract; with peptide
Predicted band size: 106 kDa
Observed band size: 106 kDa -
Immunohistochemistical detection of GABA B Receptor 2/GABBR2 antibody (ab52248) in PFA fixed Rat Dorsal root ganglion sections. ab52248 was used at 1/300 and incubated for 60 hours @ 4°C in PBS-T 0.3%. Secondary antibody: anti IgG Rabbit Conjugation: Alexa Fluor® 488 (1/1000).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab52248 は 8 報の論文で使用されています。
- Dasdelen MF et al. A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality via Regulating Brain Electrochemical Activity. Front Nutr 9:874254 (2022). PubMed: 35449538
- Li K et al. Long Noncoding RNA H19 Induces Neuropathic Pain by Upregulating Cyclin-Dependent Kinase 5-Mediated Phosphorylation of cAMP Response Element Binding Protein. J Pain Res 13:2113-2124 (2020). PubMed: 32903807
- Tabassum S et al. Jujuboside A prevents sleep loss-induced disturbance of hippocampal neuronal excitability and memory impairment in young APP/PS1 mice. Sci Rep 9:4512 (2019). PubMed: 30872728
- Sarveazad A et al. Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury. Stem Cell Res Ther 10:183 (2019). PubMed: 31234929
- Fan J et al. Metformin produces anxiolytic-like effects in rats by facilitating GABAA receptor trafficking to membrane. Br J Pharmacol 176:297-316 (2019). PubMed: 30318707
- Li H et al. Low Cerebral Exposure Cannot Hinder the Neuroprotective Effects of Panax Notoginsenosides. Drug Metab Dispos 46:53-65 (2018). PubMed: 29061584
- Li Y et al. Validation and bioinformatic analysis of propofol-induced differentially expressed microRNAs in primary cultured neural stem cells. Gene 664:90-100 (2018). WB . PubMed: 29679758
- Thibault K et al. Molecular mechanisms underlying the enhanced analgesic effect of oxycodone compared to morphine in chemotherapy-induced neuropathic pain. PLoS One 9:e91297 (2014). WB, IHC-Fr, IHC-FrFl ; Rat . PubMed: 24618941