Anti-GABA A Receptor alpha 1 抗体 (ab33299)
Key features and details
- Rabbit polyclonal to GABA A Receptor alpha 1
- Suitable for: IHC-P, WB, IP
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-GABA A Receptor alpha 1 antibody
GABA A Receptor alpha 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GABA A Receptor alpha 1 -
由来種
Rabbit -
特異性
This antibody is specific for the GABA A Receptor a1 subunit. -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Rat
交差が予測される動物種: Cow, Cynomolgus monkey -
免疫原
Synthetic peptide:
QPSQDELKDNTTVFT-C
, corresponding to N terminal amino acids 1-15 of the mature Rat GABA A Receptor alpha 1 containing a cysteine at the C terminus for linkage to a carrier protein. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
1/10000. Predicted molecular weight: 52 kDa.
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IP |
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate.
|
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/10000. Predicted molecular weight: 52 kDa. |
IP
Use at an assay dependent concentration. 5ul immunoprecipitates the GABA A Receptor a1 subunit in rat brain microsomal preparation RIPA lysate. |
ターゲット情報
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機能
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
関連疾患
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
細胞内局在
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 29705 Rat
- SwissProt: P08219 Cow
- SwissProt: P62813 Rat
- Unigene: 28463 Rat
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別名
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
画像
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Anti-GABA A Receptor alpha 1 antibody (ab33299) at 1/10000 dilution + rat brain microsomal preparation tissue lysate at 10 µg
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
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Immunohistochemistry analysis of formalin fixed paraffin embedded rat hippocampus tissue prepared using heat-induced epitope retrieval (HIER) labeling GABA A Receptor alpha 1 using ab33299 at 1/500 dilution. Secondary antibody was an anti-rabbit antibody and HRP-DAB.
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Immunoprecipitation analysis: 1 mg of RIPA lysate of rat brain microsomes
was immunoprecipitated with 5 uL of ab33299. Arrow indicates GABAA
Receptor a1 (~50kD).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab33299 は 40 報の論文で使用されています。
- Widmark A et al. ADAR1- and ADAR2-mediated regulation of maturation and targeting of miR-376b to modulate GABA neurotransmitter catabolism. J Biol Chem 298:101682 (2022). PubMed: 35124003
- Kalinowski D et al. Expression of Calbindin, a Marker of Gamma-Aminobutyric Acid Neurons, Is Reduced in the Amygdala of Oestrogen Receptor β-Deficient Female Mice. J Clin Med 11:N/A (2022). PubMed: 35407369
- Fang W et al. Activation of the GABA-alpha receptor by berberine rescues retinal ganglion cells to attenuate experimental diabetic retinopathy. Front Mol Neurosci 15:930599 (2022). PubMed: 36017075
- Choi C et al. Analyzing the mechanisms that facilitate the subtype-specific assembly of γ-aminobutyric acid type A receptors. Front Mol Neurosci 15:1017404 (2022). PubMed: 36263376
- Du T et al. Deep brain stimulation of the anterior nuclei of the thalamus relieves basal ganglia dysfunction in monkeys with temporal lobe epilepsy. CNS Neurosci Ther 27:341-351 (2021). PubMed: 33085171