Application
Flow Cytometry
Sample
Rat Cell (Lung tissue)
Permeabilization
Yes - eBiosciences FoxP3 intracellular staining buffer set
Gating Strategy
Leukocytes
Specification
Lung tissue
Preparation
Cell harvesting/tissue preparation method: Lungs were dissected and digested using Collagenase IV+DNase
Sample buffer: GKN+10% FCS
Sample buffer: GKN+10% FCS
Fixation
eBiosciences FoxP3 intracellular staining buffer set
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: eBiosciences intra-staining perm buffer
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jeff Lauzon-Joset
Verified customer
投稿 Jan 12 2016