Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Other product details
Dilution
1/3000
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Amersham
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
Negative control
GBL RNAi removes 35KDa band.
Additional data
Additional Notes
This Ab produces relatively high background (non-specific bands) compared to other Ab I routinely use with the same protocol. It does recognize GBL with enough specificity for common use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Stephen Dann
Verified customer
投稿 Dec 20 2005