Anti-GNAS 抗体 (ab101736)
Key features and details
- Goat polyclonal to GNAS
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GNAS antibody
GNAS 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to GNAS -
由来種
Goat -
特異性
ab101736 is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human G protein alpha S aa 78-91 (internal sequence).
Sequence:C-QAARSNSDGEKATK
Database link: NP_000507.1 -
ポジティブ・コントロール
- WB: KNRK cell lysate; human cerebellum and mouse and rat brain tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab101736 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101736の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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関連性
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 281793 Cow
- Entrez Gene: 2778 Human
- Entrez Gene: 14683 Mouse
- Entrez Gene: 100049657 Pig
- Entrez Gene: 24896 Rat
- Omim: 139320 Human
- SwissProt: P04896 Cow
- SwissProt: P63091 Dog
see all -
別名
- Adenylate cyclase stimulating G alpha protein antibody
- AHO antibody
- Alternative gene product encoded by XL exon antibody
see all
画像
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Lane 1 : Anti-GNAS antibody (ab101736) at 0.5 µg/ml
Lanes 2-3 : Anti-GNAS antibody (ab101736) at 0.1 µg/ml
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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Anti-GNAS antibody (ab101736) at 0.3 µg/ml + KNRK cell lysate at 35 µg
Predicted band size: 46 kDaLysates in RIPA buffer. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab101736 は 1 報の論文で使用されています。
- Müller FE et al. Serotonin receptor 4 regulates hippocampal astrocyte morphology and function. Glia 69:872-889 (2021). PubMed: 33156956