Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Cell lysate - whole cell (PC-12 cells)
Specification
PC-12 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tbst
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-rabbit IgG Horseradish peroxidase linked
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Positive control
Rat Brain
Additional data
Abcam response
This antibody has not been tested in Rat before. It is inconclusive as to whether it will work with this species or not.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 17 2013