Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Breast carcinoma)
Specification
Breast carcinoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% non fat milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 90 kDa Non-specific: 45, 25 kDa
Exposure
30 second(s)
Additional data
Additional Notes
Antibody works well but additional bands appear. Have used it several times with clear bands also showing up at 45 and 25kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 19 2014