Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Blastocyst Day7)
Permeabilization
Yes - 0,2% Triton X
Specification
Blastocyst Day7
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0,02M PBS + 0,5% Serum
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L), Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Fixative concentration was 4%. The incubation with the primary antibody was overnight at 4°C. The ab109429/AF555 detection is in green, staining of the cell nuclei with SiR-Hoechst (1:500) in red.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Irene Röttinger
Verified customer
投稿 Nov 30 2016