Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain slices)
Permeabilization
Yes - Triton 0.2%
Specification
Brain slices
Blocking step
PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.05% + Goat Serum 10%
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 647 goat anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Additional data
Additional Notes
Very clean signal with little background despite having not perfusion-fixed the tissue.
Slides were post-fixed in a 4% PFA in PBS solution for 30 minutes at 4¯C. A heat-induced antigen retrieval step was included, using a pH 6 citrate buffer solution. 0.3M Glycine was added in the blocking solution to reduce PFA-related autofluorescence. All antibody incubations included 10% goat serum.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 21 2017