Anti-Firefly Luciferase 抗体 (ab21176)
Key features and details
- Rabbit polyclonal to Firefly Luciferase
- Suitable for: ICC/IF, WB
- Reacts with: Firefly
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Firefly Luciferase antibody
Firefly Luciferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Firefly Luciferase -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Firefly -
免疫原
Full length native protein (purified) (Firefly (Photinus pyralis)).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21176の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (4) |
Use a concentration of 10 µg/ml.
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WB | (3) |
1/1000 - 1/2000.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
1/1000 - 1/2000. |
ターゲット情報
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関連性
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
細胞内局在
Peroxisome -
参照データベース
- SwissProt: P08659 Firefly
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別名
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
画像
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ab21176 at 10 µg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF.
The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody.
Left panel: Un-transfected cells.
Right panel: Transfected cells.
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ab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF.
The cells were fixed in 2% paraformaldehyde and 0.1% Triton X-100 was used for cell permeabilization (15 minutes incubation time). The cells were incubated with the antibody overnight at 4°C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel).
The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.
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All lanes : Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution
Lane 1 : Lysates from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase
Lane 2 : Lysates from HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate -
Immunocytochemical analysis analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase labeling Luciferase with ab21176 at a concentration of 10 μg/mL. The secondary antibody was a Goat anti-Rabbit IgG, FITC conjugate.
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ab21176 staining mouse mammary carcinoma cells by ICC/IF.
Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor® 555 conjugated goat anti-rabbit antibody was used as the secondary.
The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (73)
ab21176 は 73 報の論文で使用されています。
- Lee M et al. A Novel Piggyback Strategy for mRNA Delivery Exploiting Adenovirus Entry Biology. Viruses 14:N/A (2022). PubMed: 36298724
- Gredic M et al. iNOS Deletion in Alveolar Epithelium Cannot Reverse the Elastase-Induced Emphysema in Mice. Cells 12:N/A (2022). PubMed: 36611917
- Hori SS et al. A mathematical model of tumor regression and recurrence after therapeutic oncogene inactivation. Sci Rep 11:1341 (2021). PubMed: 33446671
- Truong DJ et al. Non-invasive and high-throughput interrogation of exon-specific isoform expression. Nat Cell Biol 23:652-663 (2021). PubMed: 34083785
- Maric D et al. Th17 T Cells and Immature Dendritic Cells Are the Preferential Initial Targets after Rectal Challenge with a Simian Immunodeficiency Virus-Based Replication-Defective Dual-Reporter Vector. J Virol 95:e0070721 (2021). PubMed: 34287053