Application
Western blot
Sample
Mouse Tissue lysate - whole (Schwann Cell)
Loading amount
200 µg
Specification
Schwann Cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse IgG HRP Jackson Immuno Conjugatio
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 60 kDa
Positive control
Wild Type
Negative control
dKO
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 16 2012