Application
Western blot
Sample
Dog Tissue lysate - other (epidermis)
Gel Running Conditions
Reduced Denaturing (4-15)
Loading amount
1.5 µg
Specification
epidermis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 2%BSA
Dilution
1/10
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: IgG goat anti rabbit hrp
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ecl+
Exposure
30 second(s)
Bands
Specific: 230 kDa Non-specific: 12 /100.. kDa
Additional data
Abcam response
Thank you so much for testing this antibody in dog. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 26 2018