Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Sample
Human Cell lysate - whole cell (human vascular smooth muscle cells)
Specification
human vascular smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS -0,05%Tween -5%Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit Anti-Mouse Immunoglobulins Dako
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Super Signal WestDura
Bands
Specific: 55 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 04 2013