Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney)
Permeabilization
Yes - 0.1% TritonX-100
Specification
Kidney
Blocking step
Cas-Block (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody diluent (invitrogen)
Dilution
1/250
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
Thank you for your valuable feedback about the use of ab45688 in IHC-Fr, an application we have not tested before. We welcome any further feedback and AbReviews from other scientist who have used this antibody in IHC-Fr which will help us assess how well the antibody works in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 13 2017