Anti-Ferritin Heavy Chain 抗体 [EPR3005Y] (ab75972)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ferritin Heavy Chain antibody [EPR3005Y]
Ferritin Heavy Chain 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain -
由来種
Rabbit -
特異性
This antibody recognizes only the Ferritin heavy chain. -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Ferritin Heavy Chain aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P02794 -
ポジティブ・コントロール
- WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast and tonsil tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3005Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75972の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 2495 Human
- Omim: 134770 Human
- SwissProt: P02794 Human
- Unigene: 524910 Human
- Unigene: 645560 Human
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別名
- Apoferritin antibody
- Cell proliferation inducing gene 15 protein antibody
- Cell proliferation-inducing gene 15 protein antibody
see all
画像
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All lanes : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from 1% SDS Hot lysis method.
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from RIPA lysis method
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). -
ab75972 staining Ferritin Heavy Chain in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Lanes 1-3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)
Lane 1 : Human liver lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Lanes 3-4 : Human brain lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 21 kDa -
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa -
Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in Human kidney tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab75972 showing positive staining in normal liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab75972 showing positive staining in normal breast tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Unpurified ab75972 showing positive staining in normal tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab75972 は 28 報の論文で使用されています。
- Yao W et al. Inhibition of the NADPH Oxidase Pathway Reduces Ferroptosis during Septic Renal Injury in Diabetic Mice. Oxid Med Cell Longev 2022:1193734 (2022). PubMed: 35265258
- Sun Q et al. Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5'AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells. Bioengineered 13:13269-13279 (2022). PubMed: 35635082
- Nakai Y et al. 5‑Aminolevurinic acid inhibits the proliferation of bladder cancer cells by activating heme synthesis. Oncol Rep 48:N/A (2022). PubMed: 36082808
- Li ZJ et al. Artesunate synergizes with sorafenib to induce ferroptosis in hepatocellular carcinoma. Acta Pharmacol Sin 42:301-310 (2021). PubMed: 32699265
- Yamashita A et al. Pathological Features of Ruptured Coronary Plaque and Thrombus Interfaces: Fibrin and von Willebrand Factor as Platelet Scaffolds on Rupture Sites. Thromb Haemost 121:234-241 (2021). PubMed: 32920806