Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
Synthetic peptide corresponding to Human Fbxw7 aa 533-582 (C terminal). Synthetic peptide corresponding to a region within C terminal amino acids 533-582 (LKTGEFIRNL VTLESGGSGG VVWRIRASNT KLVCAVGSRN GTEETKLLVL) of Human Fbxw7 (NP_001013433; UniProt ID Q969H0-4 isoform 3). Sequence:
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 2.5 µg/ml.
Use a concentration of 0.25 µg/ml. Predicted molecular weight: for isoform 4, 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Isoform 1 is widely expressed. Isoform 4 is expressed in brain.
Contains 1 F-box domain. Contains 7 WD repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Fbxw7 with ab105752 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Fbxw7. Right - Merge.
ICC/IF image of ab105752 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105752 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Fbxw7 antibody (ab105752)
Western blot analysis of 721_B whole cell lysate labeling Fbxw7 with ab105752 at 1.0µg/ml.
Zhang X et al. CaMKIV-dependent preservation of mTOR expression is required for autophagy during lipopolysaccharide-induced inflammation and acute kidney injury. J Immunol193:2405-15 (2014).
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