Anti-FbxL12 抗体 (ab96831)
Key features and details
- Rabbit polyclonal to FbxL12
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FbxL12 antibody -
製品の詳細
Rabbit polyclonal to FbxL12 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide containing a sequence corresponding to a region within amino acids 315-326 (
RACPKESMDWWM
) of Human FbxL12 (NP_060173). -
ポジティブ・コントロール
- HepG2 and NIH-3T3 whole cell lysates; SW480; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96831の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/500.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
|
特記事項 |
---|
IHC-P
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
ターゲット情報
-
機能
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
配列類似性
Contains 1 F-box domain.
Contains 8 LRR (leucine-rich) repeats. - Information by UniProt
-
参照データベース
- Entrez Gene: 54850 Human
- Entrez Gene: 30843 Mouse
- Entrez Gene: 313782 Rat
- Omim: 609079 Human
- SwissProt: Q9NXK8 Human
- SwissProt: Q9EPX5 Mouse
- Unigene: 12439 Human
- Unigene: 24608 Mouse
-
別名
- F box and leucine rich repeat protein 12 antibody
- F box protein FBL12 antibody
- F box/LRR repeat protein 12 antibody
see all
画像
-
Anti-FbxL12 antibody (ab96831) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS-PAGE -
Anti-FbxL12 antibody (ab96831) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 37 kDa
12% SDS-PAGE -
ab96831, at 1/500 dilution, staining FbxL12 in paraffin-embedded SW480 by Immunohistochemistry.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab96831 は 2 報の論文で使用されています。
- Zhao B et al. Notch and the pre-TCR coordinate thymocyte proliferation by induction of the SCF subunits Fbxl1 and Fbxl12. Nat Immunol 20:1381-1392 (2019). PubMed: 31451788
- Tsuruta F et al. SCFFbl12 Increases p21Waf1/Cip1 Expression Level through Atypical Ubiquitin Chain Synthesis. Mol Cell Biol 36:2182-94 (2016). WB ; Human . PubMed: 27215384