Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Sample
Pig Cell lysate - whole cell (smooth muscle cell)
Specification
smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: RABBIT IgG (H&L) Antibody IRDye800 Conjugated Pre
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/10000
Detection
Detection method
LI-COR
Bands
Specific: 42 kDa Non-specific: 45, 55 kDa
Exposure
6 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 29 2014