Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
35 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: odyssey blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 275 kDa Non-specific: 50 kDa
Positive control
Liver whole tissue lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Ahmed Nayef
Verified customer
投稿 Dec 26 2012