Fatty Acid Oxidation Complete Assay Kit (ab222944)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Purified mitochondria, Suspension cells
製品の概要
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製品名
Fatty Acid Oxidation Complete Assay Kit
Fatty Acid Oxidation キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells, Purified mitochondria -
アッセイタイプ
Cell-based (quantitative) -
製品の概要
Fatty Acid Oxidation Complete Assay Kit (ab222944) offers a complete solution to measure Fatty Acid Oxidation (FAO) in live cells using conventional fluorescence plate readers. This product combines Fatty Acid Oxidation Assay (ab217602) and Extracellular Oxygen Consumption Assay (ab197243) in one practical kit for single purchase.
The assay uses the 18C unsaturated fatty acid Oleate as substrate, and includes two FAO modulators, etomoxir and FCCP. Etomoxir, an inhibitor of the carnitine transporter CPT1, prevents Oleate import and thereby limits the supply of reducing equivalents to the ETC, reducing oxygen consumption in turn. The remaining ETC (electron transport chain) activity is driven by non-long chain FAO. FCCP treatment induces maximal ETC activity by dissipating the mitochondrial membrane potential, while the increased demand for reducing equivalents causes a concomitant increase in the FAO activity. If exogenous long-chain fatty acid is unavailable or import is inhibited, FAO activity will be limited.
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特記事項
Learn more about the full range of assays to measure glycolysis, oxygen consumption, fatty acid oxidation and metabolic flux in live cells.
Or review the full metabolism assay guide for other assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 55 tests Etomoxir 1 vial Extracellular O2 Consumption Reagent 1 vial FAO Conjugate 1 x 1ml FAO Control 1 x 500µl FAO Tablet 1 tablet FCCP 1 vial High Sensitivity Oil 1 x 15ml L-Carnitine 1 vial -
研究分野
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別名
- Beta Oxidation
- Beta-Oxidation
関連製品
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Related Products
画像
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FAO-driven oxygen respiration in HepG2 cells treated with the CPT-1 inhibitor Etomoxir (white) and uncoupler FCCP (gray).
Untreated cells (basal FAO) curve shows a steady increase of the Extracellular O2 Consumption Reagent signal reflecting ETC-driven oxygen consumption. Signal Control shows probe signal in the absence of cell respiration. Etomoxir treatment prevents oleate import, resulting in reduced availability of reducing equivalents and a resultant decrease in ETC activity. The remaining ETC activity (difference between Etoxomir treatment and Signal Control) is driven by metabolic activity other than long chain FAO. FCCP treatment induces maximal ETC activity by dissipating the mitochondrial membrane potential. Increased demand for reducing equivalents causes a concomitant increase in FAO as indicated by the rapid increase in Extracellular O2 Consumption Reagent signal. This strong increase in ETC activity is not observed where exogenous LCFA is unavailable or where import is inhibited.
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The figure summarizes the balance between these parameters under “Basal” and “Maximum” (FCCP treated) conditions. The increased energy demand imposed by FCCP treatment is met by increased FAO.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab222944 は 9 報の論文で使用されています。
- Pham DV & Park PH Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogramming. J Exp Clin Cancer Res 41:9 (2022). PubMed: 34986886
- Zhang T et al. MicroRNA-377-3p inhibits hepatocellular carcinoma growth and metastasis through negative regulation of CPT1C-mediated fatty acid oxidation. Cancer Metab 10:2 (2022). PubMed: 35057851
- Chen W et al. Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis. Cell Death Dis 13:863 (2022). PubMed: 36220838
- McKelvey KJ et al. Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma. Front Oncol 11:633210 (2021). PubMed: 33854970
- Wang B et al. Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4. J Lipid Res 62:100091 (2021). PubMed: 34118252
- Li W et al. Transcription-Based Multidimensional Regulation of Fatty Acid Metabolism by HIF1a in Renal Tubules. Front Cell Dev Biol 9:690079 (2021). PubMed: 34277635
- Zhang Y et al. Enhancing Fatty Acids Oxidation via L-Carnitine Attenuates Obesity-Related Atrial Fibrillation and Structural Remodeling by Activating AMPK Signaling and Alleviating Cardiac Lipotoxicity. Front Pharmacol 12:771940 (2021). PubMed: 34899326
- Godlewski G et al. Targeting Peripheral CB1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis. Cell Metab 29:1320-1333.e8 (2019). PubMed: 31105045
- Chen J et al. Hypoxia exacerbates nonalcoholic fatty liver disease via the HIF-2a/PPARa pathway. Am J Physiol Endocrinol Metab 317:E710-E722 (2019). PubMed: 31430204