Application
Western blot
Sample
Bos taurus Purified protein (Protein found within commercial Milk Fat Globule M)
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Loading amount
1 µg
Specification
Protein found within commercial Milk Fat Globule M
Blocking step
Seablock - thermo fisher as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea BLOCK
Dilution
1/500
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: ECL Anti-Rabbit IgG Horseradish
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Fempto
Exposure
5 minute(s) and 0 second(s)
Positive control
LC-MS/MS ran on product confirming presence of protein
Additional data
Abcam response
Thank you for submitting your feedback for this untested species. We will continue to gather data on use in cow. You may wish to try using reduced samples, as this is how we have tested other samples using WB. We appreciate that you have shared your data with us and other scientists.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 02 2017