Anti-Fast Myosin Skeletal Heavy chain 抗体 [MY-32] (ab51263)
Key features and details
- Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain
- Suitable for: ICC, IHC-P
- Reacts with: Rabbit
- Isotype: IgG1
製品の概要
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製品名
Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]
Fast Myosin Skeletal Heavy chain 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MY-32] to Fast Myosin Skeletal Heavy chain -
由来種
Mouse -
アプリケーション
適用あり: ICC, IHC-Pmore details -
種交差性
交差種: Rabbit
交差が予測される動物種: Mouse, Rat, Chicken, Guinea pig, Cow -
免疫原
Full length native protein (purified) corresponding to Rabbit Fast Myosin Skeletal Heavy chain. Purified rabbit muscle myosin.
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ポジティブ・コントロール
- ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612). IHC: Rabbit tongue section
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特記事項
This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lot is from ascites and is still in stock as of 18th October 2016 (GR255796). Lot numbers higher than GR255796 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
May be used for detecting cross striated muscle differentiation in tumors.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
Purified from Tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
MY-32 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51263の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 1 - 5 µg/ml.
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IHC-P | (4) |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Muscle contraction. -
配列類似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
ドメイン
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
細胞内局在
Cytoplasm > myofibril. Thick filaments of the myofibrils. - Information by UniProt
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参照データベース
- Entrez Gene: 417309 Chicken
- Entrez Gene: 281337 Cow
- Entrez Gene: 17879 Mouse
- Entrez Gene: 287408 Rat
- SwissProt: Q9BE40 Cow
- SwissProt: Q5SX40 Mouse
- Unigene: 477065 Mouse
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別名
- adult 1 antibody
- MYH1 antibody
- MYH1_HUMAN antibody
see all
画像
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Immunofluorescence staining of Fast Myosin Skeletal Heavy chain using ab51263 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.
The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab51263 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab51263 gave comparable results using 4% formaldehyde fixation (10 min).
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Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Rabbit tongue sections labeling Fast Myosin Skeletal Heavy chain with ab51263 at 20 μg/mL followed by Goat Anti-Mouse IgG (Fab specific)-FITC antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (66)
ab51263 は 66 報の論文で使用されています。
- Cefis M et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
- Chi MY et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
- Li S et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
- Greene MA et al. Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile. BMC Genomics 23:595 (2022). PubMed: 35971074
- Sun M et al. Research Note: Creatine monohydrate alleviates protein breakdown induced by corticosterone via inhibiting ubiquitin proteasome pathway in chicken myotubes. Poult Sci 101:102177 (2022). PubMed: 36194918