Anti-Fas 抗体 (ab110021)
Key features and details
- Goat polyclonal to Fas
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fas antibody
Fas 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Fas -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Macaque monkey -
免疫原
Synthetic peptide corresponding to Human Fas aa 150-250 (internal sequence). Synthetic peptide:
KTCRKHRKENQGSH
, corresponding to internal sequence amino acids 197-210 of Human Fas -
ポジティブ・コントロール
- Human small intestine tissue, Human tonsil tissue, MOLT4 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110021の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 38 kDa.
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IHC-P |
Use a concentration of 3.75 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 38 kDa. |
IHC-P
Use a concentration of 3.75 µg/ml. |
ターゲット情報
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機能
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
組織特異性
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
関連疾患
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
ドメイン
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
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別名
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
画像
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ab110021, staining CD95 in Human small intestine tissue by immunohistochemistry.
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ab110021, staining CD95 in Human tonsil tissue by immunohistochemistry.
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Anti-Fas antibody (ab110021) at 0.1 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab110021 は 2 報の論文で使用されています。
- Yang H et al. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways. World J Gastrointest Oncol 11:377-392 (2019). PubMed: 31139308
- Huang W et al. Inhibition of Fas associated phosphatase 1 (Fap1) facilitates apoptosis of colon cancer stem cells and enhances the effects of oxaliplatin. Oncotarget 9:25891-25902 (2018). PubMed: 29899829