Application
Western blot
Sample
Human Cell lysate - whole cell (HDMEC)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HDMEC
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 28°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 28°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL Pro
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 62 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 06 2015