Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample
Mouse Tissue sections (E9.5 mouse embryo section)
Specification
E9.5 mouse embryo section
Permeabilization
Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 26°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Image shows section of E9.5 mouse embryo, DAPI, antibody staining and overlay respectively. Good cytoplasmic staining is found in the neural tube as predicted.
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投稿 Jan 28 2015