Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cell line)
Loading amount
20000 cells
Specification
LNCaP prostate cancer cell line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/800
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Tween TBS
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti Goat polyclonal
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/500
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 200, 40, kDa
Positive control
Transient expression of FXR1 constructs 3 isoforms to detectable by WB
Negative control
siRNA knockdown of FXR1 (2 sets of oligos to target the portein) and observed the decreased level of FXR1 bands
Additional data
Additional Notes
WB membrane: Hybond-C Amersham
This antibody also works
This antibody also works
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 09 2009