Anti-FSTL1/FRP 抗体 (ab111969)
Key features and details
- Rabbit polyclonal to FSTL1/FRP
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FSTL1/FRP antibody
FSTL1/FRP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FSTL1/FRP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Cow, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Human Plasma Total Protein lysate as well as the following whole cell lysates: A431; MD MBA 231; HeLa; NIH3T3.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111969の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 35 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 35 kDa). |
ターゲット情報
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機能
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
組織特異性
Overexpressed in synovial tissues from rheumatoid arthritis. -
配列類似性
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 534482 Cow
- Entrez Gene: 11167 Human
- Entrez Gene: 14314 Mouse
- Entrez Gene: 100172767 Orangutan
- Entrez Gene: 79210 Rat
- Omim: 605547 Human
- SwissProt: Q58D84 Cow
- SwissProt: Q12841 Human
see all -
別名
- Anti FSTL1 antibody
- FLJ50214 antibody
- FLJ52277 antibody
see all
画像
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All lanes : Anti-FSTL1/FRP antibody (ab111969) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 11 kDa (possible non-specific binding), 48 kDa (possible cleavage fragment), 95 kDa (possible non-specific binding)
Exposure time: 2 minutesFSTL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The band observed at 48 kDa could potentially be a cleaved form of FSTL1 due to the presence of a 20amino acid signal peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab111969 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab111969 は 1 報の論文で使用されています。
- Wang WL et al. The lncRNA PVT1/miR-590-5p/FSTL1 axis modulates the proliferation and migration of airway smooth muscle cells in asthma. Autoimmunity 54:138-147 (2021). PubMed: 33825599